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inverted fluorescence microscope  (Carl Zeiss)


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    Structured Review

    Carl Zeiss inverted fluorescence microscope
    Inverted Fluorescence Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 98/100, based on 983 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/axio+observer+fluorescence+microscope/Inverted+microscope+Axio+Observer+7/pmc13049910-113-17-20
    Average 98 stars, based on 983 article reviews
    inverted fluorescence microscope - by Bioz Stars, 2026-10
    98/100 stars

    Images

    Related Articles

    Fluorescence:

    Article Title: Super-enhancer PPP1R15B/EIF2A axis characterizes aggressive drug-tolerant persister cells in Glioblastoma.
    Article Snippet: Coverslips were then mounted using Fluoromount-G® (SouthernBiotech, Cat. No. 0100–01) and sealed onto microscope slides. .. Fluorescent images were captured using a Zeiss Axio Observer fluorescence microscope equipped with appropriate filter sets. ..

    Article Title: Live-cell imaging of DNA damage and cell cycle progression uncovers distinct responses during neural differentiation of hiPSCs.
    Article Snippet: .. Fluorescence was visualized using an Axio Observer fluorescence microscope (Zeiss). .. Irradiation Cells were X-ray-irradiated at 2 Gy with an MBR-1520R-4 system (Hitachi Ltd.) operated at 150 kV, with a 20-mA tube current and a 0.5-mm Al + 0.3-mm Cu filter.

    Article Title: Protamine expression in somatic cells condenses chromatin and disrupts transcription without altering DNA methylation
    Article Snippet: .. Cells were stained with primary and secondary antibodies (Supplemental Table 2), followed by DAPI staining and imaged with a Zeiss Axio Observer fluorescence microscope, as described before [ ]. ..

    Article Title: Pro-Inflammatory Molecules Implicated in Multiple Sclerosis Divert the Development of Human Oligodendrocyte Lineage Cells
    Article Snippet: After fixation, cells were stained with corresponding secondary antibodies (1:400) conjugated with Alexa Fluor 647 (Thermo Fisher Scientific, Mississauga ON). .. Plates were imaged with a 10X objective using a Zeiss Axio Observer fluorescence microscope (Carl Zeiss Canada, Toronto, ON) or the ImageXpress (Molecular Devices, San Jose, CA) highcontent imaging platform after staining for O4 and PI. .. Cells were counted by a blinded individual using ImageJ software or automatically using ImageXpress software.

    Article Title: Cryo-ET of actin cytoskeleton and membrane structure in lamellipodia formation using optogenetics
    Article Snippet: .. Images were obtained using a Zeiss Axio observer fluorescence microscope (Zeiss) equipped with the ApoTome 2 module, 63×/1.4 NA oil objective lenses, and ZEN software (Zeiss). .. The Colibri 7 light source and Axio Cam 506 camera (Zeiss) were used for imaging.

    Article Title: Tailoring a novel colorectal cancer stem cell-targeted therapy by inhibiting the SMYD3/c-MYC axis.
    Article Snippet: Slides were sealed using ProLongTM Diamond Antifade Mountant (P36961, Thermo Fisher Scientific). .. Images were acquired using a Zeiss Axio Observer fluorescence microscope (Carl Zeiss). .. Primary antibodies used: SMYD3 (12859, Cell Signaling Technology, 1:100 dilution), CD44 (3570, Cell Signaling Technology, 1:200 dilution), and c-MYC K158/K163Me (custom made, project code 2ZG1940M, Thermo Fisher Scientific, 1:100 dilution), FITC-conjugated CD44 (75122, Cell Signaling Technology, 1:100 dilution), FITC-conjugated EpCAM (MA1-10197, Thermo Fisher Scientific, 1:50 dilution), PEconjugated CD133 (12-1338-42, Thermo Fisher Scientific, 1:100 dilution).

    Article Title: Tailoring a novel colorectal cancer stem cell-targeted therapy by inhibiting the SMYD3/c-MYC axis.
    Article Snippet: .. Digital image acquisition was carried out with a Zeiss Axio Observer fluorescence microscope (Carl Zeiss) using a 10x magnification objective. .. Quantification of cell death induction in tumorspheres was performed by analyzing the intensity of the red signal using ZEN blue software 3.3 version (Carl Zeiss).

    Article Title: Epigenetic regulation and mechanobiological adaptation in tenocytes during maturation
    Article Snippet: .. Phase-contrast images of cells and fluorescence images of embedded 0.2- μ m-diameter microspheres (Invitrogen, F8810) were acquired using a Zeiss Axio Observer fluorescence microscope (40× magnification). ..

    Microscopy:

    Article Title: Super-enhancer PPP1R15B/EIF2A axis characterizes aggressive drug-tolerant persister cells in Glioblastoma.
    Article Snippet: Coverslips were then mounted using Fluoromount-G® (SouthernBiotech, Cat. No. 0100–01) and sealed onto microscope slides. .. Fluorescent images were captured using a Zeiss Axio Observer fluorescence microscope equipped with appropriate filter sets. ..

    Article Title: Live-cell imaging of DNA damage and cell cycle progression uncovers distinct responses during neural differentiation of hiPSCs.
    Article Snippet: .. Fluorescence was visualized using an Axio Observer fluorescence microscope (Zeiss). .. Irradiation Cells were X-ray-irradiated at 2 Gy with an MBR-1520R-4 system (Hitachi Ltd.) operated at 150 kV, with a 20-mA tube current and a 0.5-mm Al + 0.3-mm Cu filter.

    Article Title: Protamine expression in somatic cells condenses chromatin and disrupts transcription without altering DNA methylation
    Article Snippet: .. Cells were stained with primary and secondary antibodies (Supplemental Table 2), followed by DAPI staining and imaged with a Zeiss Axio Observer fluorescence microscope, as described before [ ]. ..

    Article Title: Pro-Inflammatory Molecules Implicated in Multiple Sclerosis Divert the Development of Human Oligodendrocyte Lineage Cells
    Article Snippet: After fixation, cells were stained with corresponding secondary antibodies (1:400) conjugated with Alexa Fluor 647 (Thermo Fisher Scientific, Mississauga ON). .. Plates were imaged with a 10X objective using a Zeiss Axio Observer fluorescence microscope (Carl Zeiss Canada, Toronto, ON) or the ImageXpress (Molecular Devices, San Jose, CA) highcontent imaging platform after staining for O4 and PI. .. Cells were counted by a blinded individual using ImageJ software or automatically using ImageXpress software.

    Article Title: Cryo-ET of actin cytoskeleton and membrane structure in lamellipodia formation using optogenetics
    Article Snippet: .. Images were obtained using a Zeiss Axio observer fluorescence microscope (Zeiss) equipped with the ApoTome 2 module, 63×/1.4 NA oil objective lenses, and ZEN software (Zeiss). .. The Colibri 7 light source and Axio Cam 506 camera (Zeiss) were used for imaging.

    Article Title: Tailoring a novel colorectal cancer stem cell-targeted therapy by inhibiting the SMYD3/c-MYC axis.
    Article Snippet: Slides were sealed using ProLongTM Diamond Antifade Mountant (P36961, Thermo Fisher Scientific). .. Images were acquired using a Zeiss Axio Observer fluorescence microscope (Carl Zeiss). .. Primary antibodies used: SMYD3 (12859, Cell Signaling Technology, 1:100 dilution), CD44 (3570, Cell Signaling Technology, 1:200 dilution), and c-MYC K158/K163Me (custom made, project code 2ZG1940M, Thermo Fisher Scientific, 1:100 dilution), FITC-conjugated CD44 (75122, Cell Signaling Technology, 1:100 dilution), FITC-conjugated EpCAM (MA1-10197, Thermo Fisher Scientific, 1:50 dilution), PEconjugated CD133 (12-1338-42, Thermo Fisher Scientific, 1:100 dilution).

    Article Title: Tailoring a novel colorectal cancer stem cell-targeted therapy by inhibiting the SMYD3/c-MYC axis.
    Article Snippet: .. Digital image acquisition was carried out with a Zeiss Axio Observer fluorescence microscope (Carl Zeiss) using a 10x magnification objective. .. Quantification of cell death induction in tumorspheres was performed by analyzing the intensity of the red signal using ZEN blue software 3.3 version (Carl Zeiss).

    Article Title: Epigenetic regulation and mechanobiological adaptation in tenocytes during maturation
    Article Snippet: .. Phase-contrast images of cells and fluorescence images of embedded 0.2- μ m-diameter microspheres (Invitrogen, F8810) were acquired using a Zeiss Axio Observer fluorescence microscope (40× magnification). ..

    Staining:

    Article Title: Protamine expression in somatic cells condenses chromatin and disrupts transcription without altering DNA methylation
    Article Snippet: .. Cells were stained with primary and secondary antibodies (Supplemental Table 2), followed by DAPI staining and imaged with a Zeiss Axio Observer fluorescence microscope, as described before [ ]. ..

    Article Title: Pro-Inflammatory Molecules Implicated in Multiple Sclerosis Divert the Development of Human Oligodendrocyte Lineage Cells
    Article Snippet: After fixation, cells were stained with corresponding secondary antibodies (1:400) conjugated with Alexa Fluor 647 (Thermo Fisher Scientific, Mississauga ON). .. Plates were imaged with a 10X objective using a Zeiss Axio Observer fluorescence microscope (Carl Zeiss Canada, Toronto, ON) or the ImageXpress (Molecular Devices, San Jose, CA) highcontent imaging platform after staining for O4 and PI. .. Cells were counted by a blinded individual using ImageJ software or automatically using ImageXpress software.

    Imaging:

    Article Title: Pro-Inflammatory Molecules Implicated in Multiple Sclerosis Divert the Development of Human Oligodendrocyte Lineage Cells
    Article Snippet: After fixation, cells were stained with corresponding secondary antibodies (1:400) conjugated with Alexa Fluor 647 (Thermo Fisher Scientific, Mississauga ON). .. Plates were imaged with a 10X objective using a Zeiss Axio Observer fluorescence microscope (Carl Zeiss Canada, Toronto, ON) or the ImageXpress (Molecular Devices, San Jose, CA) highcontent imaging platform after staining for O4 and PI. .. Cells were counted by a blinded individual using ImageJ software or automatically using ImageXpress software.

    Software:

    Article Title: Cryo-ET of actin cytoskeleton and membrane structure in lamellipodia formation using optogenetics
    Article Snippet: .. Images were obtained using a Zeiss Axio observer fluorescence microscope (Zeiss) equipped with the ApoTome 2 module, 63×/1.4 NA oil objective lenses, and ZEN software (Zeiss). .. The Colibri 7 light source and Axio Cam 506 camera (Zeiss) were used for imaging.



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    Image Search Results


    Synthesis and characterization of BA-HPCS@CGRP microspheres based on microfluidic fabrication. A. Fourier transform infrared spectroscopy spectra of the HPCS, 3-Carboxyphenylboronic acid (BA), and BA-HPCS. B. The hydrogel precursors appear as a liquid macroscopically before gelation. C. The hydrogels appear milky white after photo-crosslinking. D. The imaging of BA-HPCS@CGRP microspheres based on microfluidic chips: macroscopic and microscopic observations. E. Particle size distribution of BA-HPCS@CGRP microspheres. F and G. Representative scanning electron microscope images of BA-HPCS@CGRP microspheres. H. The pore size distribution of lyophilized BA-HPCS@CGRP microspheres. I. The releasing of CGRP from BA-HPCS@CGRP in PBS and different glucose conditions (100 mg/dL, 400 mg/dL). J. Representative live/dead fluorescence images of L929 cells after co-culture with microspheres (green calcein-AM for live cells, red propidium iodide for dead cells). K. The quantitative analysis of L929 cell viability co-cultured with microspheres. ns, no significance. ∗∗∗ p < 0.001; ∗∗ p < 0.01; ∗ p < 0.05; ns, no significance. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Journal: Materials Today Bio

    Article Title: Sustained-release CGRP microspheres accelerate diabetic wound healing by synergistically promoting neurovascular regeneration through modulation of macrophage and endothelial cell functions

    doi: 10.1016/j.mtbio.2026.103015

    Figure Lengend Snippet: Synthesis and characterization of BA-HPCS@CGRP microspheres based on microfluidic fabrication. A. Fourier transform infrared spectroscopy spectra of the HPCS, 3-Carboxyphenylboronic acid (BA), and BA-HPCS. B. The hydrogel precursors appear as a liquid macroscopically before gelation. C. The hydrogels appear milky white after photo-crosslinking. D. The imaging of BA-HPCS@CGRP microspheres based on microfluidic chips: macroscopic and microscopic observations. E. Particle size distribution of BA-HPCS@CGRP microspheres. F and G. Representative scanning electron microscope images of BA-HPCS@CGRP microspheres. H. The pore size distribution of lyophilized BA-HPCS@CGRP microspheres. I. The releasing of CGRP from BA-HPCS@CGRP in PBS and different glucose conditions (100 mg/dL, 400 mg/dL). J. Representative live/dead fluorescence images of L929 cells after co-culture with microspheres (green calcein-AM for live cells, red propidium iodide for dead cells). K. The quantitative analysis of L929 cell viability co-cultured with microspheres. ns, no significance. ∗∗∗ p < 0.001; ∗∗ p < 0.01; ∗ p < 0.05; ns, no significance. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Article Snippet: Following the 30 - min incubation in the dark, images were collected with the ZEISS inverted fluorescence microscope (Carl Zeiss AG, Oberkochen, Germany; Model: Axio Observer 7).

    Techniques: Fourier Transform Infrared Spectroscopy, Spectroscopy, Imaging, Microscopy, Pore Size, Fluorescence, Co-Culture Assay, Cell Culture